hsp70 protein level  (Boster Bio)

Journal: Microbiology Spectrum

Article Title: Wine Yeast Cells Acquire Resistance to Severe Ethanol Stress and Suppress Insoluble Protein Accumulation during Alcoholic Fermentation

doi: 10.1128/spectrum.00901-22

Figure Lengend Snippet: Wine and laboratory yeast cells responded similarly to ethanol under laboratory conditions. Cells were prepared and exposed to stress, as described in the legend of Fig. 1 . The expression levels of Hsp70 (white bars) and Hsp104 (black bars) were assayed using Western blotting. Each value is expressed as the mean ± SE of the fold changes in the protein levels relative to cells without stress treatment ( n = 3).

Article Snippet: Hsp104 and Hsp70 protein levels were monitored by Western blotting using an anti-Hsp104 (ADI-SPA-1040-D; Enzo Life Sciences, Inc., Farmingdale, NY, USA), anti-Hsp70 (SMC-162c; StressMarq Biosciences, Inc., Victoria, BC, Canada), anti-mouse IgG, horseradish peroxidase (HRP)-linked (7076S; Cell Signaling Technology, Beverly, MA, USA), and anti-rabbit IgG, HRP-linked (7074S; Cell Signaling Technology) antibodies.

Techniques: Expressing, Western Blot

Journal: Microbiology Spectrum

Article Title: Wine Yeast Cells Acquire Resistance to Severe Ethanol Stress and Suppress Insoluble Protein Accumulation during Alcoholic Fermentation

doi: 10.1128/spectrum.00901-22

Figure Lengend Snippet: Hsp70 and Hsp104 levels in wine yeast cells during fermentation were assayed using Western blotting. Samples were prepared as described in the legend of Fig. 3 . As controls, log-phase cells cultured in SD medium were treated without stress (E0), exposed to 10% (vol/vol) ethanol for 180 min (E10), or pretreated with 6% ethanol for 180 min and then exposed to 10% (vol/vol) ethanol for 180 min (E6/10) in SD medium. Each value is expressed as the mean ± SE of fold changes in protein levels relative to E0.

Article Snippet: Hsp104 and Hsp70 protein levels were monitored by Western blotting using an anti-Hsp104 (ADI-SPA-1040-D; Enzo Life Sciences, Inc., Farmingdale, NY, USA), anti-Hsp70 (SMC-162c; StressMarq Biosciences, Inc., Victoria, BC, Canada), anti-mouse IgG, horseradish peroxidase (HRP)-linked (7076S; Cell Signaling Technology, Beverly, MA, USA), and anti-rabbit IgG, HRP-linked (7074S; Cell Signaling Technology) antibodies.

Techniques: Western Blot, Cell Culture

Journal: Cell & Bioscience

Article Title: Heat shock protein 70 protects mouse against post-infection irritable bowel syndrome via up-regulating intestinal γδ T cell’s Th17 response

doi: 10.1186/s13578-018-0237-z

Figure Lengend Snippet: Protein and mRNA expression of HSP 70. a , b Expression of HSP 70 detected by Western blot; c , d Expression of HSP 70 detected by RT-PCR. *p < 0.05 compared with control group; △, p < 0.05 compared with both HSP70 group and IP-IBS group

Article Snippet: HSP70 protein level was measured by Western blot (Wuhan Boster Coporation, Wuhan, China).

Techniques: Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction, Control

Journal: Cell & Bioscience

Article Title: Heat shock protein 70 protects mouse against post-infection irritable bowel syndrome via up-regulating intestinal γδ T cell’s Th17 response

doi: 10.1186/s13578-018-0237-z

Figure Lengend Snippet: γδ T cells’ Th17 response and IL-17 production by γδ T cells. a Purified γδ T cells were stained with Anti-TCRγ/δ-FITC antibodies and runned on FACS; b γδ T cells’ proliferation index was measured; c Annexin V-FITC/PI apoptosis Kit was used to determine the apoptosis rate; d The surface molecular expression of CD62L and CD69 was detected by FACS; e The production of IL17 by γδ T cells stimulated by HSP70 was measured by ELISA

Article Snippet: HSP70 protein level was measured by Western blot (Wuhan Boster Coporation, Wuhan, China).

Techniques: Purification, Staining, Expressing, Enzyme-linked Immunosorbent Assay